3DHISTECH Ltdarticle

Teleconsultation Offline teleconsultationWith E-School you get a powerful tool to help you in your teaching duties.This is another function that virtual microscopy has compared to traditional microscopy.In routine practice, different results can be obtained using different stainings.Tissue microarrays (TMA) represent a fairly new invention in routine pathology, a solution you can use effectively only with virtual microscopy.Sometimes viewing a specimen in two dimensions is not enough.With fluorescent microscopy a whole new way is opened in the diagnostic process.Virtual microscopy is based upon digital data.


 


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Scanning speed


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Scanning speed



Imaging technology


Tile based scanning has some serious advantages compared to other scanning techniques. One of them is the commercially available imaging technology. Industrial digital cameras have a very big market with several competing companies which means development is rapid. Today's digital cameras provide much higher framerate and/or higher quality and/or resolution than yesterday's and there is still much headroom for development. The Pannoramic digital slide scanners operate with these commercially available digital cameras which can be exchanged quite easily and in a matter of minutes: upgrade is really not a problem.
3DHISTECH offers two new camera options for a tremendous upgrade in speed and/or quality.

Zeiss AxioCam MRm Rev.3
This monochrome camera has a resolution of 1.4 MP with big pixel size (6.45 um x 6.45 um). This results in very high sensitivity which is essential for FL scanning. More than that, the camera has a 12 bit output which enables us to use the digital gain for even faster scanning.

The following pictures demonstrate the effect of digital gain. The slide was scanned with an AxioCam and a 20x/0.8 Plan-Apochromat objective from Zeiss. On the pictures you can see the red channel only.

Click on the small pictures for high resolution
0x_gain 2x_gain 4x_gain
  0x digital gain 2x digital gain 4x digital gain
Properties 3 FL channel specimen, scanned with MIRAX SCAN, Zeiss AxioCam MRm, Zeiss Plan-Apochromat 20x/0.8
View: red channel, at 40x magnification
Exposure time (ms) 43 12 3


As you can see the image gets a little blurred when using higher digital gain. On the other hand, we experience very low exposure times.

Next: Brightfield scanning developments