Scanning speed
Brightfield scanning developments
The 3CCD Hitachi HV-F22CL camera allows us to greatly increase the quality of MRXS slides. Using the 1.0x camera adapter and a 20x/0.8 Plan-Apochromat objective we reach a pixel resolution of 0.23 um/pixel which is better than most of our competitors' at 40x.
This major leap in image quality can be used a different way, too. By changing the camera adapter we can find a good balance between quality and speed.
For example if we use the Hitachi camera with a 0.63x camera adapter it is 2-3 times faster than with the 1.0x adapter while still maintaining a perfect image quality (pixel resolution is 0.37 um/pixel at 20x and that is still much better than that of our competitors'). This is the recommended set.
We can go even further, though. When using a 0.5x camera adapter the pixel resolution at 20x is at 0.465 um/pixel which is comparable to the competitors. With this setting we achieve a really fast scanning speed. We recommend this set primarily for routine pathology.
Below you can see some sample images and scanning data with the Hitachi camera using different camera adapters.
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Scanned area: 2.26 cm2 (see the marked area on the right)
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Click on the small pictures to open in larger size |
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Camera adapter |
1.0x |
0.63x |
0.5x |
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Pixel resolution at 20x (um / pixel) |
0.23 |
0.37 |
0.465 |
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Scanning time (min:sec) |
06:04 |
02:23 |
02:06 |
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For extra high quality scanning (FISH or hematology) a 40x objective might be necessary. With the 1.0x camera adapter we achieve an unprecedented 0.12 um/pixel resolution and with the 0.63x adapter 0.18 um/pixel. Of course this magnification slows down the scanning process considerably.
| Click on the small pictures to open in larger size |
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| Camera adapter |
1.0x |
0.63x |
| Pixel resolution at 40x (um / pixel) |
0.12 |
0.18 |
| Scanning time (min:sec) |
45:42 |
10:27 |
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